The woman accused of orchestrating an ambush attack that killed one Lake County deputy and injured two others pleaded not guilty to a list of charges, including murder, according to court records.

The murder suspect involved in the deadly Lake County Sheriff’s Office deputy ambush Friday night was detained using the fallen deputy’s handcuffs, the Lake County Sheriff’s Office released on Tuesday.

On Tuesday morning, 48-year-old Julie Jefferson Sulpizio, made her first court appearance in Lake County while on suicide watch at the Lake County jail.

One deputy was killed and two others were injured during an ambush attack Friday night in Lake County. Here is a timeline of the night and the days following the attack.

The Eustis woman who faces multiple charges, including first-degree murder on a law enforcement officer, made her first appearance in Lake County.

Three days after three Lake County sheriff’s deputies were ambushed inside a home near Eustis, investigators tell News 6 they have reached out to federal agencies to help find out more about the family they believe was behind the attack.

A Eustis woman who was taken into custody under the Baker Act following the deadly ambush of Lake County deputies was arrested on Monday afternoon, according to the Lake County Sheriff’s Office.